P-115: The Effect of Intra-Peritoneal Administration of Papaver Bracteatum Lindl. Extract on Development of NMRI Mice Oocytes Treated with Doxorubicin

Background: In this study the effect of water-alcohol Papaver bracteatum Lindl. Extract on development of mice oocytes treated with doxorubicin (dox) was examined. Materials and Methods: Mice at 7-8 weeks old were assigned to 4 groups. Control group, mice injected intraperitoneally (IP) with saline. P.bracteatum extract group alone, mice treated with 200 mg/kg of body weight, IP, twelve consecutive days. Dox group alone, mice were given dox, IP, 10 mg/kg of body weight. Experimental group, mice treated with P.bracteatum extract and doxorubicin together. Effect of the extract on development of mice oocytes treated with dox was evaluated through assisted reproductive technology techniques (ARTs). Ovulated MII oocytes were isolated from the mice oviductal ampullae. The oocytes were transferred into Human Tubal Fluid (HTF) medium supplemented with 4mg/ml BSA and capacitated sperm were added to IVF medium. After 4-6 hours, 2PN were transferred into KSOM medium supplemented with 4 mg/ml BSA. After IVF process, embryos were cultured and their developmental process was monitored for 96 hours. Results: The results of this study indicated that developmental rate and blastocyst formation improved by using of the extract. We observed a significant increase in vitro developmental competence experimental group in comparison with dox group alone (p<0.05). Conclusion: Papaver bracteatum extract, as a medicinal plant with anti-oxidant property, increases oocyte quality. Antioxidative properties of some phytochemicals of the extract are concluded as probable reasons of the extract effects. The purpose of the present study was to assess the doxorubicin toxicity to these follicles and oocytes could be prevented by extract and cause improved embryo development which obtained from mice treated with dox plus papaver compared dox alone. This study was designed to examine the possible ameliorating action of the water-alcohol Papaver bracteatum extract, on doxorubicin- induced detrimental effects in mouse models and imply extract to be a promising adjuvant agent that may attenuate the toxicity of doxorubicin.